2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Mea masani e faʻaaogaina i le piʻo faʻatulagaina o le tufatufaina atu o le tele o mole mole: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Meafaigaluega ma masini
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
I le tulaga lautele, o le pasene o amino acids i oloa a le Sustar e maualuga atu nai lo oloa a le Zinpro.
Vaega 8 Aafiaga o le fa'aaogaina
A'afiaga o punaoa eseese o minerale laiti i le fa'atinoga o le gaosiga ma le lelei o fuamoa o moa fafine i le vaitaimi mulimuli o le fofoa.
Faiga o le Gaosiga
Tekonolosi chelation fa'atatau
Tekonolosi emulsification sele
Tekinolosi fa'asusu ma fa'amago i le mamafa
Tekinolosi fa'amālūlū ma fa'amamago
Tekonolosi fa'atekonolosi fa'aleleia atili mo le puleaina o le siosiomaga
Fa'aopoopoga A: Metotia mo le Fuafuaina o le tufatufaina atu o le mamafa o mole mole o peptides
Fa'aaogaina o tulaga fa'atonuina: GB/T 22492-2008
1 Mataupu Faavae o le Su'ega:
Sa fuafuaina e ala i le chromatography fa'amamāina gel maualuga le faatinoga. O lona uiga, o le fa'aaogaina o le porous filler e fai ma vaega tumau, e fa'avae i luga o le eseesega i le tele o le molecular mass o vaega fa'ata'ita'i mo le vavae'eseina, na iloa i le peptide bond o le ultraviolet absorption wavelength o le 220nm, fa'aaoga le polokalama fa'apitoa mo le fa'atinoina o fa'amatalaga mo le fuafuaina o le tufatufaina atu o le molecular mass e ala i le gel filtration chromatography (e pei o le polokalama GPC), o chromatograms ma a latou fa'amatalaga na fa'agasoloina, fuafuaina e maua ai le tele o le molecular mass o le soybean peptide ma le lautele o le tufatufaina atu.
2. Mea Fa'aola
E tatau i le vai faʻataʻitaʻi ona ausia le faʻamatalaga o le vai lona lua i le GB/T6682, o le faʻaaogaina o reagents, vagana ai ni aiaiga faapitoa, e mama faʻapitoa.
2.1 O mea e fa'aaogaina e aofia ai le acetonitrile (mama i le chromatographic), trifluoroacetic acid (mama i le chromatographic),
2.2 Mea masani e faʻaaogaina i le piʻo faʻatulagaina o le tufatufaina atu o le tele o mole mole: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Meafaigaluega ma masini
3.1 Chromatograph Liquid Fa'atinoga Maualuga (HPLC): o se nofoaga faigaluega chromatographic po'o se mea e tu'ufa'atasia ai ma se masini e iloa ai le UV ma se polokalama fa'agasolo fa'amaumauga GPC.
3.2 Iunite e fa'amamā ma aveese ai le kasa mai le masini e fa'aaogā ai le vacuum mo le feavea'i.
3.3 Paleni eletise: tau fa'asolosolo 0.000 1g.
4 Laasaga o le Fa'agaioiga
4.1 Tulaga o le chromatographic ma faʻataʻitaʻiga o le fetuʻunaʻiga o le faiga (tulaga faʻasino)
- 4.1.1 Koluma fa'a-chromatographic: TSKgelG2000swxl300 mm×7.8 mm (lautele i totonu) po'o isi koluma gel o le ituaiga lava e tasi ma le fa'atinoga tutusa e talafeagai mo le fuafuaina o porotini ma peptides.
- 4.1.2 Vaega feavea'i: Acetonitrile + vai + trifluoroacetic acid = 20 + 80 + 0.1.
- 4.1.3 Gafataulima o le iloa: 220 nm.
- 4.1.4 Saosaoa o le tafe: 0.5 mL/min.
- 4.1.5 Taimi e iloa ai: 30 minute.
- 4.1.6 Fa'ata'ita'iga o le tele o tui: 20μL.
- 4.1.7 Vevela o le koluma: vevela o le potu.
- 4.1.8 Ina ia ausia e le faiga o le chromatographic manaoga o le iloa, na faatulagaina ai i lalo o tulaga o le chromatographic o loʻo i luga, o le lelei o le koluma o le gel chromatographic, o lona uiga, o le numera faʻateorē o papatusi (N), e le itiiti ifo i le 10000 na fuafuaina e faʻavae i luga o tumutumuga o le tulaga faʻatulagaina o le tripeptide (Glycine-Glycine-Glycine).
- 4.2 Gaosiga o pi'o masani o le mamafa fa'a-molekula fa'atatau
- O vailaʻau faʻatulagaina eseese o le peptide mole mole mole eseese o loʻo i luga ma le maualuga o le mamafa o le 1 mg / mL na saunia e ala i le faʻafetaui o le vaega feaveaʻi, faʻafefiloi i se vaega patino, ona faʻamamā lea e ala i le membrane vaega faʻaola ma le tele o le pu o le 0.2 μm ~ 0.5 μm ma tuiina i totonu o le faʻataʻitaʻiga, ona maua ai lea o chromatograms o tulaga faʻatulagaina. O piʻo faʻatulagaina o le mamafa mole mole ma a latou faʻatusatusaga na maua e ala i le faʻatulagaina o le logarithm o le mamafa mole mole faʻatatau i le taimi e taofia ai poʻo le faʻasologa laina.
4.3 Togafitiga fa'ata'ita'i
Fuafua lelei le 10mg o le faʻataʻitaʻiga i totonu o se fagu voluma 10mL, faʻaopopo sina vaega feaveaʻi, lūlūina i le ultrasonic mo le 10 minute, ina ia liu suavai atoa le faʻataʻitaʻiga ma palu faʻatasi, faʻavaivaia i le vaega feaveaʻi i le fua, ona faʻamamā lea i totonu o se membrane organic phase e iai le tele o pu o le 0.2μm~0.5μm, ma suʻesuʻeina le filtrate e tusa ai ma tulaga chromatographic i le A.4.1.
- 5. Fuafuaina o le tufatufaina atu o le mamafa o le mole mole
- A maeʻa ona iloiloina le fofo faʻataʻitaʻiga na saunia i le 4.3 i lalo o tulaga chromatographic o le 4.1, e mafai ona maua le mamafa molecular faʻatatau o le faʻataʻitaʻiga ma lona lautele tufatufaina e ala i le suia o faʻamatalaga chromatographic o le faʻataʻitaʻiga i totonu o le calibration curve 4.2 i le polokalama faʻagasologa faʻamaumauga GPC. O le tufatufaina o mamafa molecular faʻatatau o peptides eseese e mafai ona fuafuaina e ala i le metotia o le peak area normalization, e tusa ai ma le fua faʻatatau: X=A/A aofaʻi × 100
- I le fua fa'atatau: X - O le vaega tele o se peptide tele mole mole fa'atatau i le aofa'i atoa o le peptide i le fa'ata'ita'iga, %;
- A - O le tumutumuga o le vaega o se peptide e tele ai mole mole;
- Aofa'i A - o le aofa'i o vaega pito i luga o peptide ta'itasi o le mamafa mole mole, ua fuafuaina i le tasi le numera tesimale.
- 6 Toe Faifaipea
- O le eseesega atoatoa i le va o fuafuaga tutoatasi e lua na maua i lalo o tuutuuga o le toe faia e le tatau ona sili atu i le 15% o le averesi fa'a-aritematika o fuafuaga e lua.
- Fa'aopoopoga B: Metotia mo le Fuafuaina o Amino Acids Sa'oloto
- Fa'aaogaina o le tulaga fa'atonuina: Q/320205 KAVN05-2016
- 1.2 Mea fa'aola ma meafaitino
- Asiteka aisa: mama fa'ata'ita'i
- Asitala perchloric: 0.0500 mol/L
- Fa'ailoga: 0.1% fa'ailoga violē tioata (aseta acetic glacial)
- 2. Fuafuaina o amino acids saoloto
Sa fa'amago fa'ata'ita'iga i le 80°C mo le 1 itula.
Tuu le faʻataʻitaʻiga i totonu o se pusa mago e faʻamālūlū ai i le vevela masani o le potu pe faʻamālūlū ifo i se vevela e mafai ona faʻaaogaina.Fuafua pe tusa ma le 0.1 kalama o le fa'ata'ita'iga (sa'o i le 0.001 kalama) i totonu o se fagu fa'aputu mago e 250 mL.Fa'avave ona agai atu i le isi la'asaga e 'alofia ai le mitiia e le fa'ata'ita'iga o le susu o le si'osi'omagaFa'aopopo le 25 mL o le glacial acetic acid ma fa'afefiloi lelei mo le sili atu i le 5 minute.Fa'aopoopo ni mataua se 2 o le fa'ailoga vaiolē tioataFa'afefiloi i le 0.0500 mol / L (±0.001) o le vaila'au fa'atulagaina o le perchloric acid se'ia o'o ina suia le vaila'au mai le lanu violē i le i'uga.
Faamaumau le aofaʻi o le vai faʻasolo ua faʻaaogaina.
- Faatino le suega gaogao i le taimi e tasi.
- 3. Fuafuaga ma taunuuga
- O le aofaʻi o amino acid saoloto X i totonu o le reagent e faʻaalia o se vaega tele (%) ma e fuafuaina e tusa ai ma le fua faʻatatau: X = C × (V1-V0) × 0.1445/M × 100%, i le fua faʻatatau:
- C - Fa'aputuga o le vaila'au masani o le perchloric acid i moles i le lita (mol/L)
- V1 - Voluma e fa'aaogaina mo le titration o fa'ata'ita'iga i le vaila'au masani o le perchloric acid, i milliliters (mL).
- Vo - Voluma e fa'aaogaina mo le titration blank i le vaila'au masani o le perchloric acid, i milliliters (mL);
M - Mamafa o le faʻataʻitaʻiga, i kalama (g).
| 0.1445: O le mamafa masani o amino acids e tutusa ma le 1.00 mL o le vailaʻau masani o le perchloric acid [c (HClO4) = 1.000 mol / L]. | 4.2.3 Vai fa'a-titration masani a le Cerium sulfate: fa'aputuga c [Ce (SO4) 2] = 0.1 mol/L, saunia e tusa ai ma le GB/T601. | |
| Fa'aaogaina o tulaga fa'atonuina: Q/70920556 71-2024 | 1. Mataupu faavae o le fuafuaina (Fe o se faataitaiga) | E matuā maualalo lava le solubility o amino acid iron complexes i le anhydrous ethanol ae o free metal ions e mafai ona solubility i le anhydrous ethanol, o le eseesega i le solubility i le va o ia mea e lua i le anhydrous ethanol na faʻaaogaina e fuafua ai le fua faatatau o le chelation o amino acid iron complexes. |
| I le fua fa'atatau: V1 - le aofa'i o le vai fa'atulagaina o le cerium sulfate ua fa'aaogaina mo le titration o le vai fa'ata'ita'i, mL; | Etanolo e leai se vai; o le vaega o totoe e tutusa ma le fuaiupu 4.5.2 i le GB/T 27983-2011. | 3. Laasaga o le suʻesuʻega |
| Fai ni tofotofoga se lua i le taimi e tasi. Fuafua le 0.1g o le faʻataʻitaʻiga ua faʻamago i le 103±2℃ mo le 1 itula, saʻo i le 0.0001g, faʻaopopo le 100mL o le ethanol e leai se vai e faʻataʻapeʻape ai, faʻamama, fufuluina toega o le faʻamama i le 100mL o le ethanol e leai se vai mo le itiiti ifo ma le faatolu, ona faʻaliliu lea o toega i totonu o se fagu conical 250mL, faʻaopopo le 10mL o le vailaʻau sulfuric acid e tusa ai ma le fuaiupu 4.5.3 i le GB/T27983-2011, ona faʻatino lea o laʻasaga nei e tusa ai ma le fuaiupu 4.5.3 "Faʻavevela e faʻataʻapeʻape ai ona tuʻu lea e malulu" i le GB/T27983-2011. Faʻatino le suega gaogao i le taimi e tasi. | 4. Fuafuaina o le aofaʻi atoa o le uʻamea | 4.1 O le mataupu faavae o le fuafuaina e tutusa ma le fuaiupu 4.4.1 i le GB/T 21996-2008. |
4.2. Mea Fa'aola ma Vaifofo
| 4.2.1 'Aseta fefiloi: Fa'aopopo le 150mL o le 'aseta sulfuric ma le 150mL o le 'aseta phosphoric i le 700mL o le vai ma fa'afefiloi lelei. | 4.2.2 Vai fa'ailoga o le sodium diphenylamine sulfonate: 5g/L, saunia e tusa ai ma le GB/T603. | 4.2.3 Vai fa'a-titration masani a le Cerium sulfate: fa'aputuga c [Ce (SO4) 2] = 0.1 mol/L, saunia e tusa ai ma le GB/T601. | |
| 4.3 Laasaga o le su'esu'ega | Fai ni tofotofoga se lua i le taimi e tasi. Fuafua le 0.1g o le faʻataʻitaʻiga, saʻo i le 020001g, tuʻu i totonu o se fagu faʻaputu e 250mL, faʻaopopo le 10mL o le vailaʻau faʻafefiloi, a uma ona faʻafefiloi, faʻaopopo le 30ml o le vai ma le 4 mataua o le vailaʻau faʻailoga sodium dianiline sulfonate, ona faʻatino lea o laʻasaga nei e tusa ai ma le fuaiupu 4.4.2 i le GB/T21996-2008. Faʻatino le suega gaogao i le taimi e tasi. | 4.4 Fa'atusa o i'uga | O le aofaʻi atoa o le uʻamea X1 o le amino acid iron complexes e tusa ai ma le vaega tele o le uʻamea, o le tau o loʻo faʻaalia i le %, na fuafuaina e tusa ai ma le fua faʻatatau (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - vai fa'asolo o le cerium sulfate ua fa'aaogaina mo le titration o le vai fa'asolo, mL; | V0 - vai fa'asolo o le cerium sulfate ua fa'aaogaina mo le titration o le vai fa'asolo, mL; | C - O le maualuga moni o le vaifofo masani o le cerium sulfate, mol/L5. Fuafuaina o le aofaʻi o le uʻamea i totonu o chelatesO le aofaʻi o le uʻamea X2 i le chelate e tusa ai ma le vaega tele o le uʻamea, o le tau o loʻo faʻaalia i le %, na fuafuaina e tusa ai ma le fua faʻatatau: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| I le fua fa'atatau: V1 - le aofa'i o le vai fa'atulagaina o le cerium sulfate ua fa'aaogaina mo le titration o le vai fa'ata'ita'i, mL; | V2 - vai fa'asolo o le cerium sulfate ua fa'aaogaina mo le titration o le vai fa'asolo, mL;nom1-Mamafa o le faʻataʻitaʻiga, g. Ia avea le averesi faʻa-arithmetic o taunuuga o le fuafuaina tutusa ma taunuuga o le fuafuaina, ma o le eseesega atoatoa o taunuuga o le fuafuaina tutusa e le sili atu i le 0.3%. | 0.05585 - o le mamafa o le u'amea u'amea ua fa'aalia i le kalama e tutusa ma le 1.00 mL o le vai fa'avae o le cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1-Mamafa o le faʻataʻitaʻiga, g. Ia avea le averesi faʻa-arithmetic o taunuuga o le fuafuaina tutusa ma taunuuga o le fuafuaina, ma o le eseesega atoatoa o taunuuga o le fuafuaina tutusa e le sili atu i le 0.3%. | 6. Fuafuaina o le fua faatatau o le chelationFua faatatau o le Chelation X3, o le tau o loʻo faʻaalia i le %, X3 = X2/X1 × 100Fa'aopoopoga C: Metotia mo le Fuafuaina o le fua faatatau o le chelation a le Zinpro |
Fa'aaogaina o le tulaga fa'atonuina: Q/320205 KAVNO7-2016
1. Mea fa'aola ma meafaitino
a) Asetika aisa: mama fa'ata'ita'i; b) Asetika perchloric: 0.0500mol/L; c) Fa'ailoga: 0.1% fa'ailoga violē tioata (asetika aisa)
2. Fuafuaina o amino acids saoloto
2.1 Na fa'amago fa'ata'ita'iga i le 80°C mo le 1 itula.
2.2 Tuu le faʻataʻitaʻiga i totonu o se pusa mago e faʻamālūlū ai i le vevela masani o le potu pe faʻamālūlū ifo i se vevela e mafai ona faʻaaogaina.
2.3 Fuafua pe tusa ma le 0.1 kalama o le faʻataʻitaʻiga (saʻo i le 0.001 kalama) i totonu o se fagu faʻaputu mago e 250 mL
2.4 Fa'avave loa i le isi la'asaga e 'alofia ai le mitiia e le fa'ata'ita'iga o le susu o le si'osi'omaga.
2.5 Fa'aopopo le 25mL o le glacial acetic acid ma fa'afefiloi lelei mo le sili atu i le 5 minute.
2.6 Fa'aopoopo ni mataua se 2 o le fa'ailoga vaiolē tioata.
2.7 Fa'afefiloi i le 0.0500mol/L (±0.001) o le vaila'au fa'atulagaina o le perchloric acid se'ia o'o ina suia le vaila'au mai le violē i le lanumeamata mo le 15 sekone e aunoa ma le suia o le lanu o le i'uga.
2.8 Faamaumau le aofaʻi o le vai faʻasolo ua faʻaaogaina.
2.9 Faatino le suega avanoa i le taimi e tasi.
- 3. Fuafuaga ma taunuuga
- Katalana
- Physicochemical parameters
V1 - Voluma e fa'aaogaina mo le titration o fa'ata'ita'iga i le vaila'au masani o le perchloric acid, i milliliters (mL).
Vo - Voluma e fa'aaogaina mo le titration blank i le vaila'au masani o le perchloric acid, i milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Tuatusi: No.147 Qingpu Road, Shouan Town, Pujiang County, Chengdu City, Sichuan Province, Saina
Telefoni: 86-18880477902
Oloa
Minerale e le'o fa'aolaola
- Minerale fa'aolaola
- Swahili
- Auaunaga fa'apitoa
- So'otaga vave
Talaaga o le Kamupani
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Kiliki mo se fesili | © Puletaofia - 2010-2025: Taofia Aia Tatau Uma. | Fa'afanua o le Upega Tafa'ilagi SU'ESU'EGA SILI Telefoni |
| Telefoni | 86-18880477902 | Javanese | Imeli |
| 8618880477902 | Saina | Falani | |
| Bird | Saina | Falani | Siamani Sipaniolo |
| Aquatic animals | Iapani | Kolea | Alapi Greek |
| Turkish | Italia | ||
| Ruminant animal g/head day | January 0.75 | Indonesian Fa'a Afelika Swedish |
Polish
- Basque
- Katalana
- Physicochemical parameters
Hindi
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgarian
- Sepuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Croatian
Siamani
| Application object | Urdu Viatename | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haitian | Hausa | Kinyarwanda Hmong Hungarian |
| Piglets and fattening pigs | Igbo | Javanese | Kannada Khmer Kutisa |
| Kirikisi | Latina | ||
| Bird | 300～400 | 45～60 | Macedonian Malay Malayalam |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Norwegian
- Pasato
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbian
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahili
Tajik
Tamil
Telugu
Fa'a Thai
| Application object | Urdu Viatename | Content in full-value feed (mg/kg) | Efficacy |
| Yiddish | Yoruba | Zulu | Kinyarwanda Oriya tamaloloa Take |
| Uikaha | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
